Figure 3: (A) Schematic of the SSAW-based sorter excited by FIDTs. (B) The concentric geometry of the FIDTs. θ is the degree of arcs and R is the radius of innermost transducer. O is the focal point and the two sets of FIDTs is O-axis symmetric. (C) An optical image of our high throughput SSAW sorter.
Acoustic-based fluorescence activated cell sorters (AFACS) have drawn increased attention in recent years due to their versatility, high biocompatibility, high controllability, and simple design. We developed a high-throughput AFACS method based on standing surface acoustic waves (SSAWs) (Fig. 3). We utilized a pair of focused interdigital transducers (FIDTs) to generate SSAW with high resolution and high energy efficiency. We demonstrated a minimum actuation time of 72 μs, which translates to a potential sorting rate of more than 13,800 events per second.
References:
https://www.nsf.gov/news/news_summ.jsp?cntn_id=136443&org=NSF&from=newsField
https://nsf.gov/news/news_summ.jsp?cntn_id=137527&org=NSF&from=news
Jinjie Shi, Shahrzad Yazdi, Sz-Chin Steven Lin, Xiaoyun Ding, I-Kao Chiang, Kendra Sharpd and Tony Jun Huang, Focusing Microparticles in a Microfluidic Channel with Standing Surface Acoustic Waves (SSAW), Lab on a Chip, Vol. 8, pp. 221-223, 2008. [PDF]
Jinjie Shi, Shahrzad Yazdi, Sz-Chin Steven Lin, Xiaoyun Ding, I-Kao Chiang, Kendra Sharp and Tony Jun Huang, Three-Dimensional Continuous Particle Focusing in a Microfluidic Channel via Standing Surface Acoustic Waves (SSAW), Lab on a Chip, Vol. 11, pp. 2319-2324, 2011. (featured as front cover image) [PDF]
Xiaoyun Ding, Sz-Chin Steven Lin, Michael Ian Lapsley, Sixing Li, Xiang Guo, Chung Yu Chan, I-Kao Chiang, Lin Wang, J. Philip McCoy and Tony Jun Huang, Standing Surface Acoustic Wave (SSAW) Based Multichannel Cell Sorting, Lab on a Chip, Vol.12, pp. 4228–4231, 2012. (featured as front cover image) [PDF]
Sixing Li, Xiaoyun Ding, Feng Guo, Yuchao Chen, Michael Ian Lapsley, Sz-Chin Steven Lin, Lin Wang, J. Philip McCoy, Craig E. Cameron and Tony Jun Huang, An on-chip, multichannel droplet sorter using standing surface acoustic waves (SSAW), Analytical Chemistry, Vol. 85, pp. 5468-5474, 2013.[PDF]
Yuchao Chen, Ahmad Ahsan Nawaz, Yanhui Zhao, Po-Hsun Huang, J. Phillip McCoy, Stewart J. Levine, Lin Wang and Tony Jun Huang, Standing surface acoustic wave (SSAW)-based microfluidic cytometer, Lab on a Chip, Vol. 14, pp. 916-923, 2014.[PDF]
Ahmad Ahsan Nawaz, Yuchao Chen, Nitesh Nama, Ruth Helmus Nissly, Liqiang Ren, Adem Ozcelik, Lin Wang, J. Philip McCoy, Stewart J. Levine and Tony Jun Huang, An Acoustofluidic Fluorescence Activated Cell Sorter, Analytical Chemistry, Vol. 87, pp. 12051−12058, 2015.(featured as front cover image)[PDF]
Liqiang Ren, Yuchao Chen, Peng Li, Zhangming Mao, Po-Hsun Huang, Joseph Rufo, Feng Guo, Lin Wang, J. Philip McCoy, Stewart J. Levine, and Tony Jun Huang, A high-throughput acoustic cell sorter, Lab on a Chip, Vol. 15, pp. 3870-3879, 2015. [PDF]